Apoptosis induced by modified ribonucleosides in human cell culture systems.

Abstract

The in vitro modulation of apoptosis and cell proliferation by modified in comparison with non-modified ribonucleosides was investigated for the first time using peripheral blood lymphocytes, HL-60 cells and Caco-2 cells as human cell culture models. Modulating effects of several ribonucleosides were found in the range of 10(-7)-10(-3) mol/l. The following ribonucleosides induced significant apoptosis of HL-60 cells: adenosine, N6-dimethyladenosine, N6-(2-isopentenyl)-adenosine, N2-dimethylguanosine. A significant apoptotic effect on PBL was found with N6-dimethyladenosine and N6-(2-isopentenyl)-adenosine. N6-Dimethyladenosine, N6-(2-isopentenyl)-adenosine and guanosine had a pronounced inhibitory effect on Caco-2 cell apoptosis. Regarding the known function of ribonucleosides as pathobiochemical marker molecules for cancer, the possibility of a selective apoptotic effect against malignant cells is discussed.

• Authors: H Meisel, S Günther, D Martin, E Schlimme
• Journal: FEBS letters (FEBS Lett) Vol. 433 Issue 3 Pg. 265-8 (Aug 21 1998) ISSN: 0014-5793 [Print] England
• PMID: 9744808 (Publication Type: Journal Article)

Chemical References

• Ribonucleosides
• Guanosine
• Inosine
• Isopentenyladenosine
• Adenosine

Topics

• Adenosine (analogs & derivatives, pharmacology)
• Apoptosis (drug effects)
• Cells, Cultured
• Colonic Neoplasms
• Guanosine (analogs & derivatives, pharmacology)
• HL-60 Cells (cytology, drug effects)
• Humans
• Inosine (analogs & derivatives, pharmacology)
• Isopentenyladenosine (pharmacology)
• Kinetics
• Lymphocytes (cytology, drug effects, physiology)
• Ribonucleosides (pharmacology)
• Tumor Cells, Cultured