Orphan opioid receptor antisense probes block orphanin FQ-induced hyperphagia.

Abstract

Orphanin FQ/nociceptin binds with high affinity to the orphan opioid receptor-like/K-3 (ORL1/KOR-3) clone, and stimulates feeding. The present study demonstrated that antisense oligodeoxynucleotides directed against either exons 1, 2 or 3 of the ORL1/KOR-3 clone reduced orphanin FQ/nociceptin-induced hyperphagia. A missense probe was ineffective. Naltrexone dose-dependently reduced orphanin FQ/nociceptin-induced hyperphagia. These data suggest that the receptor responsible for orphanin FQ/nociceptin-induced hyperphagia is encoded by the ORL1/KOR-3 clone.

• Authors: L Leventhal, J P Mathis, G C Rossi, G W Pasternak, R J Bodnar
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 349 Issue 1 Pg. R1-3 (May 15 1998) ISSN: 0014-2999 [Print] Netherlands
• PMID: 9669488 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Narcotic Antagonists
• Oligonucleotides, Antisense
• Opioid Peptides
• Receptors, Opioid
• Naltrexone
• nociceptin
• Nociceptin Receptor
• Oprl protein, rat

Topics

• Animals
• Eating (drug effects)
• Exons
• Hyperphagia (chemically induced, physiopathology)
• Male
• Naltrexone (pharmacology)
• Narcotic Antagonists (pharmacology)
• Oligonucleotides, Antisense (pharmacology)
• Opioid Peptides (toxicity)
• Rats
• Rats, Sprague-Dawley
• Receptors, Opioid (genetics, physiology)
• Nociceptin Receptor