Collagenase-1 complexes with alpha2-macroglobulin in the acute and chronic wound environments.

Abstract

The purpose of this study was to examine the appearance and activation of collagenase-1 (MMP-1) in the wound environment. We found that MMP-1 accumulates in the fluid phase of the burn wound environment within 2 d of injury and reaches maximal levels by day 4. Two forms of the enzyme were evident; one that corresponded to proMMP-1 and another that corresponded to a group of high molecular mass (approximately 200 kDa and >200 kDa doublet) MMP-1 containing complexes. ProMMP-1 and MMP-1 containing complexes also occurred in wound fluid from venous stasis ulcers, but neither was detected in mastectomy fluid or in plasma. Levels of the proteinase inhibitor alpha2-macroglobulin in burn fluid and chronic ulcer wound fluid were almost as high as in plasma, and the high molecular mass MMP-1 containing complexes in burn fluid appeared to result from binding between alpha2-macroglobulin and activated MMP-1. These observations provide direct evidence that active MMP-1 in the fluid phase of the wound environment becomes complexed to alpha2-macroglobulin.

• Authors: F Grinnell, M Zhu, W C Parks
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 110 Issue 5 Pg. 771-6 (May 1998) ISSN: 0022-202X [Print] United States
• PMID: 9579544 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Culture Media, Conditioned
• alpha-Macroglobulins
• Collagenases
• Matrix Metalloproteinase 1

Topics

• Acute Disease
• Blood Physiological Phenomena
• Body Fluids (metabolism)
• Burns (metabolism)
• Chronic Disease
• Collagenases (chemistry, metabolism)
• Culture Media, Conditioned (pharmacology)
• Enzyme Activation (physiology)
• Fibroblasts (metabolism)
• Humans
• Matrix Metalloproteinase 1
• Molecular Weight
• Wounds and Injuries (metabolism)
• alpha-Macroglobulins (metabolism)