Capillary electrophoresis with electrospray mass spectrometry detection for low-molecular-mass compounds.

Abstract

Mass spectrometry (MS) detection using electrospray ionization (ESI) has been explored for the separation by capillary electrophoresis (CE) of a number of sample mixtures containing low-molecular-mass species. Optimal sheath liquid composition has been determined using a peptide mixture in which femtomolar quantities of analyte were easily observed. Effects of CE buffer choice were studied in detail. Also, a separation of basic drugs in cough syrup has been successfully detected by ESI-MS. Using negative ionization, a mixture of alkyl sulfonates and a mixture of food dyes were analyzed. All components were easily resolved and identified by molecular weight.

• Authors: T E Wheat, K A Lilley, J F Banks
• Journal: Journal of chromatography. A (J Chromatogr A) Vol. 781 Issue 1-2 Pg. 99-105 (Sep 26 1997) ISSN: 0021-9673 [Print] Netherlands
• PMID: 9368381 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Alkanesulfonates
• Antitussive Agents
• Buffers
• Coloring Agents
• Peptides

Topics

• Alkanesulfonates (analysis)
• Antitussive Agents (analysis, chemistry)
• Buffers
• Coloring Agents (analysis)
• Electrophoresis, Capillary (methods)
• Hydrogen-Ion Concentration
• Mass Spectrometry (methods)
• Molecular Weight
• Peptides (analysis, chemistry)