2-Nitropropane-induced DNA damage in rat bone marrow.

Abstract

DNA damage detected by the comet assay (single cell gel electrophoresis) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) formation in DNA in the bone marrow has been studied in groups of 6 male Wistar rats treated with a single i.p. injection of the carcinogen 2-nitropropane (2-NP, 100 mg/kg body weight) or vehicle. Twenty-four hours after 2-NP the average tail length in the comet assay in bone marrow cells was increased from 1.46 +/- 0.27 to 9.61 +/- 1.56 microm (mean /- SD, p < 0.01), and 8-oxodG levels in the DNA were increased from 1.04 +/- 0.50 to 5.14 +/- 2.42 per 10(5) dG (p < 0.01). There was a close correlation between the comet tail length and the 8-oxodG level (r = 0.89, p < 0.05). The results indicate that 2-NP inflicts DNA damage in the bone marrow cells and thus could be leukemogenic.

• Authors: X S Deng, J Tuo, H E Poulsen, S Loft
• Journal: Mutation research (Mutat Res) Vol. 391 Issue 3 Pg. 165-9 (Jul 14 1997) ISSN: 0027-5107 [Print] Netherlands
• PMID: 9268041 (Publication Type: Journal Article)

Chemical References

• Nitroparaffins
• 8-Hydroxy-2'-Deoxyguanosine
• Deoxyguanosine
• 2-nitropropane
• Propane

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Animals
• Bone Marrow (drug effects)
• DNA Damage
• Deoxyguanosine (analogs & derivatives, metabolism)
• Electrophoresis (methods)
• Male
• Mutagenicity Tests
• Nitroparaffins (metabolism, toxicity)
• Propane (analogs & derivatives, metabolism, toxicity)
• Rats
• Rats, Wistar