Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia.

Abstract

A total of 173 Vibrio cholerae O1 isolates from the recent cholera epidemic in Colombia was analysed by the polymerase chain reaction (PCR) for the genes encoding the A subunit of cholera toxin (ctxA) and the zonula occludens toxin (zot), and by ribotyping. All isolates were positive for ctx A and zot, which was confirmed by hybridisation. Ribotyping with restriction endonuclease Bg/I digestion of total DNA revealed three ribotypes: B5a comprising 165 (96.4%) isolates, and two new designated ribotypes B20 and B21a in six (3.5%) isolates and two (1.1%) isolates, respectively. These findings have significant public health implications.

• Authors: M Tamayo, S Koblavi, F Grimont, E Castañeda, P A Grimont
• Journal: Journal of medical microbiology (J Med Microbiol) Vol. 46 Issue 7 Pg. 611-6 (Jul 1997) ISSN: 0022-2615 [Print] England
• PMID: 9236746 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Endotoxins
• RNA, Bacterial
• RNA, Ribosomal
• zonula occludens toxin, Vibrio cholerae
• Cholera Toxin

Topics

• Bacterial Typing Techniques
• Cholera (epidemiology, microbiology)
• Cholera Toxin (genetics)
• Colombia (epidemiology)
• Disease Outbreaks
• Endotoxins
• Genes, Bacterial
• Humans
• Molecular Epidemiology
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• RNA, Bacterial (genetics)
• RNA, Ribosomal (genetics)
• Vibrio cholerae (classification, genetics, isolation & purification)
• rRNA Operon