Abstract | BACKGROUND & AIMS: METHODS:
GDNF and RET were detected immunohistochemically, and GDNF was quantified by immunoassay in specimens of human adult and fetal colon and Hirschsprung's disease intestine. RESULTS:
GDNF-like immunoreactivity was detected in all specimens. Immunostaining of GDNF was restricted to neural fiber-like structures across the gut wall and was similar to staining with markers of glia and Schwann cells. In contrast, RET immunoreactivity was found only in neural cell bodies. GDNF levels determined by immunoassay were higher in muscle than mucosal gut layers, and there was no difference between affected and unaffected segments of Hirschsprung's disease. CONCLUSIONS:
GDNF is present in adult and fetal human gut, where it may play a neurotrophic role. Its staining pattern suggests that it is localized in glia or Schwann cells. There seems to be no difference of GDNF levels between affected and unaffected intestinal segments in Hirschsprung's disease.
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Authors | K J Bär, P Facer, N S Williams, P K Tam, P Anand |
Journal | Gastroenterology
(Gastroenterology)
Vol. 112
Issue 4
Pg. 1381-5
(Apr 1997)
ISSN: 0016-5085 [Print] United States |
PMID | 9098026
(Publication Type: Journal Article)
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Chemical References |
- Drosophila Proteins
- GDNF protein, human
- Glial Cell Line-Derived Neurotrophic Factor
- Glial Cell Line-Derived Neurotrophic Factor Receptors
- Glial Fibrillary Acidic Protein
- MAS1 protein, human
- Nerve Growth Factors
- Nerve Tissue Proteins
- Proto-Oncogene Mas
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-ret
- Receptor Protein-Tyrosine Kinases
- Ret protein, Drosophila
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Topics |
- Adult
- Drosophila Proteins
- Fetus
(metabolism)
- Glial Cell Line-Derived Neurotrophic Factor
- Glial Cell Line-Derived Neurotrophic Factor Receptors
- Glial Fibrillary Acidic Protein
(metabolism)
- Hirschsprung Disease
(metabolism)
- Humans
- Immunoassay
- Immunohistochemistry
- Intestinal Mucosa
(metabolism)
- Intestines
(innervation)
- Nerve Fibers
(metabolism)
- Nerve Growth Factors
(metabolism)
- Nerve Tissue Proteins
(metabolism)
- Proto-Oncogene Mas
- Proto-Oncogene Proteins
(metabolism)
- Proto-Oncogene Proteins c-ret
- Receptor Protein-Tyrosine Kinases
(metabolism)
- Reference Values
|