Neutralizing and fusion-blocking antibodies induced by recombinant visna virus envelope glycoprotein.

Abstract

A recombinant envelope glycoprotein derived from visna virus, a natural lentivirus pathogen of sheep, induced antibodies that neutralized cell-free virus and blocked virus-mediated cell-to-cell fusion. The visna virus envelope gene was subcloned into a baculovirus expression vector and was expressed in insect cells. A pair of guinea pigs were immunized with the recombinant glycoprotein, and postimmunization sera neutralized cell-free visna virus infectivity and also blocked virus-mediated syncytium formation when infected and uninfected cells were cocultured together. Thus, the recombinant visna surface envelope glycoprotein is capable of inducing both neutralizing and fusion-blocking antibodies. In addition to its relevance for vaccine development, the recombinant glycoprotein will be a useful tool to study differences between antibody-mediated enhancement versus neutralization during virus-host interactions in lentivirus infections in vivo.

• Authors: D L Huso
• Journal: Viral immunology (Viral Immunol) Vol. 10 Issue 1 Pg. 15-20 ( 1997) ISSN: 0882-8245 [Print] United States
• PMID: 9095528 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Glycoproteins
• Recombinant Fusion Proteins
• Viral Envelope Proteins

Topics

• Animals
• Antibodies, Viral (immunology)
• Antigens, Viral (genetics, immunology)
• Cell Fusion
• Cell Line
• Cells, Cultured
• Glycoproteins (genetics, immunology)
• Guinea Pigs
• Macrophages (cytology, immunology)
• Neutralization Tests
• Recombinant Fusion Proteins (genetics, immunology)
• Recombination, Genetic
• Spodoptera (cytology)
• Viral Envelope Proteins (genetics, immunology)
• Visna-maedi virus (immunology)