Abstract |
Bleomycin (BLM) cytotoxicity was greatly enhanced by vortex-stirring BLM and mammalian cells for a few seconds together with 1 to 10 microg/ml high molecular weight polyacrylic acid (A-119). When suspensions of murine leukemia P388 cells were injected together with BLM and A-119 into the tail vein of CDF1 mice, cell viability was reduced to 1/1000, the reduction being similar to that obtained by vortex-stirring the cells with BLM and A-119 in vitro. This was corroborated by an increase in the survival time of these mice. The reduction in cell viability was noted only when the cells, BLM, and A-119 were simultaneously injected. There was absolutely no effect when there was a time-lag between cell inoculation and injection of BLM/A-119. These findings suggest that the conditions created by the blood stream may simulate those of vortex-stirring and that, in both cases, rapid uni-directional movement of cells with high molecular weight polyacrylic acid may affect the plasma membrane facilitating internalization of non-permeant materials into cells.
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Authors | Y Ikeda, N Shimizu, Y Kawazoe |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 20
Issue 1
Pg. 25-7
(Jan 1997)
ISSN: 0918-6158 [Print] Japan |
PMID | 9013801
(Publication Type: Journal Article)
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Chemical References |
- Acrylic Resins
- Antibiotics, Antineoplastic
- Bleomycin
- carbopol 940
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Topics |
- Acrylic Resins
(pharmacology)
- Animals
- Antibiotics, Antineoplastic
(therapeutic use)
- Bleomycin
(pharmacokinetics, therapeutic use)
- Cell Membrane Permeability
- Cells, Cultured
- Leukemia P388
(drug therapy)
- Mice
- Molecular Weight
- Neoplasm Transplantation
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