Abstract |
We investigated the expression of the yeast Kex2 family endoproteases furin and PACE4, and brain natriuretic peptide (BNP) in the atrium and ventricle after infarction as well as the conversion of the BNP precursor gammaBNP to BNP-45. In a rat heart failure model, plasma BNP rose in two phases--first at day 3, and again at day 14. BNP mRNA, as measured by Northern blot analysis, increased strongly at day 3, then at days 14 and 28 less strongly in the atrium, and in the ventricle it increased weakly at day 3, then strongly at days 14 and 28. Furin mRNA showed the same pattern of expression as that of BNP message, whereas PACE4 message stayed unchanged after the infarction. Both furin and BNP were immunostained in the myocardium adjacent to the infarcted tissue. We suggest that after myocardial infarction, furin is co-expressed with BNP in both the atrium and ventricle, and that furin may be responsible for the conversion of gammaBNP to BNP-45.
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Authors | Y Sawada, M Inoue, T Kanda, T Sakamaki, S Tanaka, N Minamino, R Nagai, T Takeuchi |
Journal | FEBS letters
(FEBS Lett)
Vol. 400
Issue 2
Pg. 177-82
(Jan 03 1997)
ISSN: 0014-5793 [Print] England |
PMID | 9001393
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nerve Tissue Proteins
- RNA, Messenger
- Natriuretic Peptide, Brain
- Endopeptidases
- Pcsk6 protein, rat
- Proprotein Convertases
- Serine Endopeptidases
- Subtilisins
- Furin
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Topics |
- Animals
- Arteries
- Blotting, Northern
- Coronary Vessels
- Endopeptidases
(metabolism)
- Furin
- Heart Atria
(metabolism)
- Heart Ventricles
(metabolism)
- Immunoenzyme Techniques
- Male
- Myocardial Infarction
- Natriuretic Peptide, Brain
- Nerve Tissue Proteins
(blood, genetics, metabolism)
- Proprotein Convertases
- Protein Processing, Post-Translational
- RNA, Messenger
- Rats
- Rats, Wistar
- Serine Endopeptidases
(genetics, metabolism)
- Subtilisins
(genetics, metabolism)
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