A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen.

Abstract

A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.

• Authors: J A House, J L Stott, M T Blanchard, M LaRocco, M E Llewellyn
• Journal: Annals of the New York Academy of Sciences (Ann N Y Acad Sci) Vol. 791 Pg. 333-44 (Jul 23 1996) ISSN: 0077-8923 [Print] United States
• PMID: 8784514 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Antigens, Viral
• Capsid Proteins
• Immunoglobulin M
• VP7 protein, Rotavirus

Topics

• African Horse Sickness (diagnosis, immunology)
• Animals
• Antibodies, Monoclonal
• Antibodies, Viral (blood)
• Antigens, Viral (immunology, radiation effects)
• Capsid (immunology, radiation effects)
• Capsid Proteins
• Cattle
• Chlorocebus aethiops
• Enzyme-Linked Immunosorbent Assay (methods)
• Equidae
• Gamma Rays
• Horses
• Immunoglobulin M (blood)
• Mice
• Mice, Inbred BALB C
• Neutralization Tests
• Orbivirus (immunology)
• Vero Cells