Toxicity of ribosome-inactivating proteins-containing immunotoxins to a human bladder carcinoma cell line.
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| Abstract |
Immunotoxins were prepared by linking the type 1 ribosome-inactivating proteins (RIP) momordin I, pokeweed antiviral protein from seeds (PAP-S) and saporin-S6 to the 48-127 monoclonal antibody (MAb) recognising a glycoprotein (gp54) expressed on all human bladder tumours tested and on human bladder carcinoma cell lines, in particular on the T24 cell line. T24 cells required a 2 hr contact with immunotoxins to ensure binding and endocytosis. A time course of exposure, followed by further incubation without the immunotoxins, showed that maximum inhibition of protein synthesis by T24 cells was reached after 2 hr of contact followed by 3 days without the immunotoxins. Under optimal conditions, 48-127/RIP immunotoxins at nanomolar concentrations inhibited by 50% protein synthesis of target T24 cells. No toxicity was observed if (i) target cells were treated with non-conjugated RIP, (ii) target cells were treated with momordin I- or PAP-S-containing immunotoxins made with an irrelevant antibody and (iii) a non-target cell line was treated with the same 2 RIP conjugated to 48-127 antibody. The in vitro selective toxicity of these immunotoxins encourages further studies in view of a possible use in clinical trials for the local therapy of human bladder carcinomas.
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| Authors | M G Battelli, L Polito, A Bolognesi, L Lafleur, Y Fradet, F Stirpe |
| Journal | International journal of cancer (Int J Cancer) Vol. 65 Issue 4 Pg. 485-90 (Feb 08 1996) ISSN: 0020-7136 [Print] United States |
| PMID | 8621232 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
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