[Construction and expression of the gene CD4V1V2-PE40, coding for a molecular targeted protein against AIDS].

Abstract

We here replaced the cell-binding domain in Pseudomonas exotoxin A (PE) with HIV-binding portion of CD4V1V2 molecule and constructed a chimeric gene CD4V1V2-PE40, which can be expressed in E. coli. The hybrid protein displays targeting toxicity toward cells infected by HIV and thus represents a promising novel therapeutic agent for the treatment of AIDS.

• Authors: X Zhao, M Zhang, H Li, S Lu
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 17 Issue 4 Pg. 248-52 (Aug 1995) ISSN: 1000-503X [Print] China
• PMID: 8575044 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• Bacterial Toxins
• CD4 Antigens
• Exotoxins
• Recombinant Fusion Proteins
• Virulence Factors
• ADP Ribose Transferases
• Pseudomonas aeruginosa exotoxin A

Topics

• ADP Ribose Transferases
• Acquired Immunodeficiency Syndrome
• Antiviral Agents (pharmacology)
• Bacterial Toxins (genetics)
• CD4 Antigens (genetics)
• Cloning, Molecular
• Escherichia coli (genetics, metabolism)
• Exotoxins (genetics)
• Gene Expression
• Humans
• Plasmids
• Pseudomonas aeruginosa
• Recombinant Fusion Proteins (biosynthesis, pharmacology)
• Simian Immunodeficiency Virus (drug effects)
• Virulence Factors