New insights into sequence recognition process of esperamicin A1 and calicheamicin gamma 1I.: origin of their selectivities and "induced fit" mechanism.

Abstract

This study addresses the DNA sequence recognition event of the enediyne antibiotics esperamicin A1 and calicheamicin gamma 1I by the use of synthetic DNA oligomers, salt effects, and circular dichroism studies. The results reported here provide several important insights: (1) esperamicin A1 requires a purine/pyrimidine trimer in host DNA for favorable interaction, (2) the sequence selectivity of esperamicin C is an origin of esperamicin A1 and calicheamicin gamma 1I selectivities, (3) in the target recognition by esperamicin C, its total structure and hydrophobicity are important, and (4) the binding of hydrophobic esperamicin to DNA duplex induces dehydration and conformational change of the host DNA. The specific sequence recognition process of esperamicin/calicheamicin has been discussed.

• Authors: M Uesugi, Y Sugiura
• Journal: Biochemistry (Biochemistry) Vol. 32 Issue 17 Pg. 4622-7 (May 04 1993) ISSN: 0006-2960 [Print] United States
• PMID: 8485139 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aminoglycosides
• Anti-Bacterial Agents
• Antibiotics, Antineoplastic
• Enediynes
• calicheamicin gamma(1)I
• DNA
• esperamicin A1

Topics

• Aminoglycosides
• Anti-Bacterial Agents (metabolism)
• Antibiotics, Antineoplastic (metabolism)
• Base Sequence
• Binding Sites
• Circular Dichroism
• DNA (chemistry, metabolism)
• Enediynes
• Molecular Sequence Data
• Nucleic Acid Conformation