Epidermal growth factor elevates the activity levels of glycolipid sulfotransferases in renal-cell-carcinoma cells.

Abstract

Accumulation of sulfoglycolipids associated with markedly elevated levels of glycolipid-sulfotransferase activity was previously demonstrated in human renal-cell-carcinoma cells. To elucidate the regulatory mechanisms of sulfoglycolipid metabolism in renal-cell carcinoma, effects of various growth factors on the sulfotransferase-activity levels were investigated using a human renal-cell-carcinoma cell line, SMKT-R3. Exogenous epidermal growth factor (EGF) significantly increased the activity levels of the sulfotransferases in a dose-dependent manner, but did not change that of arylsulfatase A, which hydrolyzes sulfoglycolipids. Furthermore, metabolic labeling with 35S-sulfate revealed that the addition of EGF to the culture medium of the cells resulted in an increment of sulfoglycolipid synthesis. The expression of the EGF receptor on SMKT-R3 cells was demonstrated by affinity cross-linking with 125I-EGF. These observations suggest that EGF can regulate sulfotransferase-activity levels in renal-cell-carcinoma cells, and function as one of the regulatory factors of sulfoglycolipid synthesis in these carcinoma cells.

• Authors: T Kobayashi, K Honke, S Gasa, N Kato, T Miyazaki, A Makita
• Journal: International journal of cancer (Int J Cancer) Vol. 55 Issue 3 Pg. 448-52 (Sep 30 1993) ISSN: 0020-7136 [Print] United States
• PMID: 8104164 (Publication Type: Journal Article)

Chemical References

• Glycolipids
• sulfoglycolipids
• Epidermal Growth Factor
• ErbB Receptors
• Sulfurtransferases
• Sulfotransferases
• galactosylceramide sulfotransferase
• Cerebroside-Sulfatase

Topics

• Carcinoma, Renal Cell (chemistry, enzymology)
• Cerebroside-Sulfatase (metabolism)
• Epidermal Growth Factor (pharmacology)
• ErbB Receptors (analysis)
• Glycolipids (metabolism)
• Humans
• Kidney Neoplasms (chemistry, enzymology)
• Sulfotransferases
• Sulfurtransferases (metabolism)
• Tumor Cells, Cultured