Abstract |
The presence of EF-Tu, RNA polymerase subunit alpha, and EF-G on the lambdadfus-3 genome and EF-Tu, ribosomal proteins L7/L12, and RNA polymerase subunit beta on the lambdadrifd 18 genome has been confirmed using a two-dimensional gel electrophoresis technique sensitive to changes in isoelectric point and molecular weight. In this system two EF-Tu gene products could not be resolved. Following infection of ultraviolet light-irradiated Escherichia coli with either lambdadfus-3 or lambdadrifd18, the EF-Tu gene, tufA, near 65 minutes on the genetic map is expressed as 3-4 copies per EF-G molecule. The EF-Tu gene, tufB, near 79 minutes on the genetic map, is expressed at about one-third of this rate. alpha is expressed as 1 copy per EF-G molecule, beta as 0.14 per EF-G molecule and L7/L12 as 2.5 per EF-G. These figures compare well with the relative amounts found in exponentially-growing cells, in which the ratio of EF-Tu to EF-G is approximately 5. Almost 90% of the total number of proteins (calculated on a molecular weight basis) which theoretically can be encoded on the lambdadrifd18 have been identified on the two-dimensional gel.
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Authors | S Pedersen, S V Reeh |
Journal | Molecular & general genetics : MGG
(Mol Gen Genet)
Vol. 144
Issue 3
Pg. 339-43
(Mar 30 1976)
ISSN: 0026-8925 [Print] Germany |
PMID | 775303
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bacterial Proteins
- Peptide Elongation Factors
- Ribosomal Proteins
- DNA-Directed RNA Polymerases
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Topics |
- Bacterial Proteins
(analysis)
- Coliphages
- DNA Viruses
- DNA-Directed RNA Polymerases
(biosynthesis)
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
(metabolism, radiation effects)
- Genotype
- Peptide Elongation Factors
- Radiation Genetics
- Ribosomal Proteins
(biosynthesis)
- Transformation, Genetic
- Ultraviolet Rays
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