Abstract |
Our earlier spin label electron paramagnetic resonance (EPR) studies of hereditary spherocytosis (HS) erythrocyte revealed the existence of structural alteration(s) when the membrane is subjected to heat stress. We have now used saturation transfer EPR to show restricted motion in membrane proteins even without subjecting membrane to stress. The abnormal motional behavior is amplified when membranes are incubated at 47 degrees C and is readily detectable by conventional EPR. Gel electrophoresis and lipid assays show that proteins but not lipids are released upon heating. Thus, the more restricted motions in HS membranes may be due to a different membrane protein organization, ultimately resulting in the abnormal morphology of HS cells.
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Authors | L W Fung, M S Ostrowski |
Journal | Life sciences
(Life Sci)
Vol. 35
Issue 20
Pg. 2071-8
(Nov 12 1984)
ISSN: 0024-3205 [Print] Netherlands |
PMID | 6092813
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Electron Spin Resonance Spectroscopy
(methods)
- Erythrocyte Membrane
(ultrastructure)
- Hot Temperature
- Humans
- Membrane Proteins
(blood)
- Spherocytosis, Hereditary
(blood)
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