[Isozymes of NADP-dependent isocitrate dehydrogenase in normal and tumor tissues of various mouse strains and their hybrids].

Abstract

Normal tissues of DBA, CBA, CC57W, C3H, Balb/c, SHR mice and F1 hybrids CC57W/DBA appeared to differ in the ratios of mitochondrial and supernatant NADP-dependent isocitrate dehydrogenase (IDH). Tested inbred mice strains CC57W, C3H, SHR, Balb/c contain allelic form Idh-1a of supernatant IDH gene Idh-1, whereas allelic form Idh-1b is characteristic of mice strains DBA and CBA. In tumors IDH isozymes have the same mobility as do isozymes of homologous normal tissues; but their activity is lower. A high variability of each isozyme activity in the isozyme spectrum is revealed in various tissues of F1 hybrids CC57W/DBA. Allelic forms of gene Idh-1 were used as markers of normal and tumor cells for the experimental model: transplantation of sarcoma 37 (Idh-1a/Idh-1a) to subcutaneous tissue of the mouse strain DBA (Idh-1b/Idh-1b). It enables us to reveal isozymes of stromal cell in tumor IDH isozyme spectrum. The results indicate that the relation of normal and tumor isozymes vary in different tumors.

• Authors: E V Fedorova, Iu B Vakhtin
• Journal: Tsitologiia (Tsitologiia) Vol. 27 Issue 7 Pg. 837-40 (Jul 1985) ISSN: 0041-3771 [Print] Russia (Federation)
• Vernacular Title: Izozimy NADF-zavisimoĭ izotsitratdegidrogenazy v normal'nykh i opukholevykh tkaniakh mysheĭ razlichnykh liniĭ i ikh gibridov.
• PMID: 4049525 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Isoenzymes
• Methylcholanthrene
• Isocitrate Dehydrogenase
• isocitrate dehydrogenase (NADP+)

Topics

• Alleles
• Animals
• Crosses, Genetic
• Electrophoresis, Polyacrylamide Gel
• Genes
• Genetic Variation
• Isocitrate Dehydrogenase (analysis, genetics)
• Isoenzymes (analysis, genetics)
• Methylcholanthrene
• Mice
• Mice, Inbred Strains (metabolism)
• Neoplasm Transplantation
• Neoplasms, Experimental (chemically induced, enzymology)
• Rhabdomyosarcoma (enzymology)