Relationship between lymphocytic cytotoxicity and phytohemagglutinin-induced lymphoblastogenesis before and after surgical tumor excision: an experimental study.

Abstract

Experimental study was performed on changes in lymphocytic cytotoxicity and phytohemagglutinin (PHA)-lymphoblastogenesis after surgical tumor excision and on a possibility of estimating antitumor immunocompetence based on the latter parameter, using 3-methyl-cholanthrene-induced mammary cancer, MRMT-1, of SD rats. When the tumor was excised at 2 weeks after inoculation, cytotoxicity showed, after a transient rise, a marked reduction at 4 weeks postoperatively in the cases of postoperative negative metastasis, while it showed a high peak at 3 weeks postoperatively, followed by a sharp descent, in the cases of postoperative positive metastasis. On the other hand, PHA-lymphoblastogenesis was found to recover a relatively high level postoperatively, in the cases of both negative and positive metastases. It appears to be difficult to estimate specific cytotoxicity from PHA-blastogenesis, but both are useful, in complementary fashion, to estimate the antitumor immunocompetence.

• Authors: S Yamamoto, K Sakata, T Kunieda, S Saji, H Tanemura, T Takekoshi, Y Sugiyama
• Journal: Journal of surgical oncology (J Surg Oncol) Vol. 28 Issue 1 Pg. 42-9 (Jan 1985) ISSN: 0022-4790 [Print] United States
• PMID: 3871496 (Publication Type: Journal Article)

Chemical References

• Phytohemagglutinins

Topics

• Animals
• Cells, Cultured
• Cytotoxicity, Immunologic
• Female
• Immunocompetence
• Lymphocyte Activation
• Mammary Neoplasms, Experimental (immunology, pathology, surgery)
• Neoplasm Metastasis
• Neoplasm Transplantation
• Organ Size
• Phytohemagglutinins (pharmacology)
• Rats
• Rats, Inbred Strains
• Spleen (cytology, immunology)
• T-Lymphocytes (drug effects, immunology)
• T-Lymphocytes, Cytotoxic (immunology)
• Thymus Gland (cytology, immunology)
• Time Factors