Thiram, an agricultural
insecticide, has been demonstrated to induce tibial
dyschondroplasia (TD) in avian species.
Circular RNA (
circRNAs), a novel class of functional
biological macromolecules characterized by their distinct circular structure, play crucial roles in various biological processes and diseases. Nevertheless, the precise regulatory mechanism underlying
non-coding RNA involvement in
thiram-induced broiler tibial chondrodysplasia remains elusive. In this study, we established a broiler model of
thiram exposure for 10 days to assess TD and obtain a
ceRNA network by
RNA sequencing. By analyzing the differentially expressed
circRNAs network, we id entify that circ_003084 was significantly upregulated in TD cartilage. Elevated circ_003084 inhibited TD chondrocytes proliferation and differentiation in vitro but promote apoptosis. Mechanistically, circ_003084 competitively binds to miR-130c-5p and prevents miR-130c-5p to decrease the level of BMPR1A, which upregulates the expression of apoptosis genes
Caspase 3,
Caspase 9, Bax and Bcl2, and finally facilitates cell apoptosis. Taken together, these findings imply that circ_003084/miR-130c-5p/BMPR1A interaction regulated TD chicken chondrocyte proliferation, apoptosis, and differentiation. This is the first work to reveal the mechanism of regulation of
circRNA-related
ceRNA on
thiram-induced TD, offering a key reference for environmental toxicology.