Inosine 5'-monophoaphate (
IMP) is a
food additive that promotes serious lipohyperplasia in the liver of C57/KsJ-db/db (db/db) mice. Thus,
IMP taken orally by healthy mice might also damage their health. To date, how
IMP affects health after being taken by healthy animals is still unclear. Therefore, we investigated the health of C57BL/6J mice affected by
IMP intake. Our data revealed that C57BL/6J mice administered 255 μM
IMP daily via oral gavage for 4 months caused
hyperlipidemia and an increase in body fat rate. The expressions of
acetyl-CoA carboxylase 1 (ACC1) and phosphorylated
acetyl-CoA carboxylase 2 (ACC2) in hepatocytes increased though the administration of
IMP, promoting the phosphorylation of
adenosine 5'-monophosphate-activated
protein kinase (AMPK). The conversion of
acetyl-CoA into
triglycerides (TGs) was promoted by ACC1. These TGs were transported from the hepatocytes to avoid the development of
non-alcoholic fatty liver disease (
NAFLD), causing a deficiency of
acetyl-CoA in the liver, and then, the increased phosphorylated ACC2 promoted the cytoplasm
fatty acids entering the mitochondria and conversion into
acetyl-CoA through the
fatty acid β-oxidation pathway, causing a deficiency in
fatty acids. Therefore, the liver showed enhanced absorption of exogenous
fatty acids, which were converted into TGs, causing lipohyperplasia. In conclusion, an excessive
IMP intake promotes metabolic dysfunction in adipose tissue.