N6-methyladenosine (m6A)-connected lncRNAs are linked to survival and immune infiltration in glioma patients.

Abstract

BACKGROUND:

The connection between m6A-assiociateed lncRNAs and prognosis has been demonstrated in multiple types of tumors. However, potential roles of m6A-assiociateed lncRNAs in glioma is still rare.

METHODS:

We implemented consensus cluster analysis to group the downloaded samples into two subtypes. The least absolute shrinkage and selection operator (LASSO) analysis was used to create a risk model. Additionally, the conjunction between m6A-related lncRNAs and immune cells infiltration was explored by conducting the R package. Ultimately, we inspected the underlying downstream pathways of the two subtypes by performing Gene Set Enrichment Analysis (GSEA). The expression level of m6A-connected lncRNAs in glioma were examined by conducting in vitro experiments.

RESULTS:

We ascertained two subtypes of glioma in line with the consensus clustering of m6A-associated lncRNAs. We confirmed that age, grade, and IDH are related to the two subtypes. Additionally, the immune cells infiltration and immune checkpoint molecules of the two clusters were discussed. A risk signature including AL359643.3, AL445524.1, AL162231.2, AL117332.1, AP001486.2, POLR2J4, AC120036.4, LINC00641, LINC00900, CRNDE, and AL158212.3, was identified using the Cox regression and LASSO analyses. We also verified the prognostic value and discussed the immune cells infiltration and immune checkpoint molecules of the risk signature. In vitro experiments verified that the m6A-associated lncRNAs was abnormally expressed in glioma.

CONCLUSION:

We elaborated the significant role of m6A-connected lncRNAs in glioma prognosis and immune infiltration and suggest that these key regulators may serve as underlying therapeutic targets to build up the efficacy of glioma immunotherapy.

• Authors: Wei Jun Wu, Feng Xiao, Yaping Xiong, Gu Feng Sun, Yun Guo, Xiang Zhou, Guo Wen Hu, Kai Huang, Hua Guo
• Journal: Bioscience reports (Biosci Rep) Vol. 43 Issue 5 (05 05 2023) ISSN: 1573-4935 [Electronic] England
• PMID: 37083719 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2023 The Author(s).

Chemical References

• RNA, Long Noncoding
• Immune Checkpoint Proteins
• Adenosine

Topics

• Humans
• RNA, Long Noncoding (genetics)
• Immune Checkpoint Proteins
• Glioma (genetics)
• Adenosine