Effects of high oxygen tension on healthy volunteer microcirculation.

Abstract

INTRODUCTION:

Previous studies have highlighted hyperoxia-induced microcirculation modifications, but few have focused on hyperbaric oxygen (HBO) effects. Our primary objective was to explore hyperbaric hyperoxia effects on the microcirculation of healthy volunteers and investigate whether these modifications are adaptative or not.

METHODS:

This single centre, open-label study included 15 healthy volunteers. Measurements were performed under five conditions: T0) baseline value (normobaric normoxia); T1) hyperbaric normoxia; T2) hyperbaric hyperoxia; T3) normobaric hyperoxia; T4) return to normobaric normoxia. Microcirculatory data were gathered via laser Doppler, near-infrared spectroscopy and transcutaneous oximetry (PtcO₂). Vascular-occlusion tests were performed at each step. We used transthoracic echocardiography and standard monitoring for haemodynamic investigation.

RESULTS:

Maximal alterations were observed under hyperbaric hyperoxia which led, in comparison with baseline, to arterial hypertension (mean arterial pressure 105 (SD 12) mmHg vs 95 (11), P < 0.001) and bradycardia (55 (7) beats·min⁻¹ vs 66 (8), P < 0.001) while cardiac output remained unchanged. Hyperbaric hyperoxia also led to microcirculatory vasoconstriction (rest flow 63 (74) vs 143 (73) perfusion units, P < 0.05) in response to increased PtcO₂ (104.0 (45.9) kPa vs 6.3 (2.4), P < 0.0001); and a decrease in laser Doppler parameters indicating vascular reserve (peak flow 125 (89) vs 233 (79) perfusion units, P < 0.05). Microvascular reactivity was preserved in every condition.

CONCLUSIONS:

Hyperoxia significantly modifies healthy volunteer microcirculation especially during HBO exposure. The rise in PtcO₂ promotes an adaptative vasoconstrictive response to protect cellular integrity. Microvascular reactivity remains unaltered and vascular reserve is mobilised in proportion to the extent of the ischaemic stimulus.

• Authors: Nicolas Cousin, Julien Goutay, Patrick Girardie, Raphaël Favory, Elodie Drumez, Daniel Mathieu, Julien Poissy, Erika Parmentier, Thibault Duburcq
• Journal: Diving and hyperbaric medicine (Diving Hyperb Med) Vol. 52 Issue 4 (Dec 20 2022) ISSN: 1833-3516 [Print] Australia
• PMID: 36525683 (Publication Type: Journal Article)
• Copyright: Copyright: This article is the copyright of the authors who grant Diving and Hyperbaric Medicine a non-exclusive licence to publish the article in electronic and other forms.

Chemical References

• Oxygen

Topics

• Humans
• Microcirculation (physiology)
• Hyperoxia
• Healthy Volunteers
• Oxygen
• Hemodynamics (physiology)
• Hyperbaric Oxygenation (methods)