Abstract |
The discovery of new effective and safe antimalarial drugs is mandatory. In this report, we formulate and evaluate transdermal (td) 1,2,6,7-tetraoxaspiro[7.11] nonadecane (N-89) using the Plasmodium berghei rodent malaria parasite in vivo model. The selected solvent for the ointment type of td N-89 was polyethylene glycol (PEG) [PEG400:PEG 4000 = 8:1 (v/w)]. We tested different application areas of 4, 6, and 8 cm2 on the shaved backs of mice. Pharmacokinetic (PK) analysis of N-89 parameters after a single 4 cm2 transdermal application revealed that the Tmax was 2 h, the T1/2 was 1.9 h, and the AUC was 1951.1 ng.h/mL. More than 10 ng/mL of plasma concentration was maintained for 12 h. The ED50 values for the 4, 6, and 8 cm2 application areas in a 4-day suppressive test were 18.9, 25.1, and 26.8 mg/kg, respectively. We additionally tested the cure effect of td N-89 in mice at a dose of 60 mg/kg, twice daily for 4 days at 0.2% parasitemia. Parasites disappeared following day 7 post-treatment in all td N-89 treated groups. Mice were cured without any parasite recurrence or dermal irritation. In conclusion, this study determined for the first time the PK parameters and effect of a new ointment type of td N-89. This suggests that transdermal treatment with N-89 is an effective and safe alternative route for the treatment of malaria, especially in children.
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Authors | Nagwa S M Aly, Hiroaki Matsumori, Thi Quyen Dinh, Akira Sato, Shin-Ich Miyoshi, Kyung-Soo Chang, Hak Sun Yu, Hye-Sook Kim |
Journal | Parasitology international
(Parasitol Int)
Vol. 93
Pg. 102720
(Apr 2023)
ISSN: 1873-0329 [Electronic] Netherlands |
PMID | 36516945
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Mice
- Animals
- Antimalarials
(pharmacology, therapeutic use)
- Ointments
(pharmacology, therapeutic use)
- Malaria
(drug therapy, parasitology)
- Plasmodium berghei
- Parasitemia
(drug therapy)
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