A low-cost, flexible extruder for liposomes synthesis and application for Murrayafoline A delivery for cancer treatment.

Abstract

Liposomal encapsulation is a drug delivery strategy with many advantages, such as improved bioavailability, ability to carry large drug loads, as well as controllability and specificity towards various targeted diseased tissues. Currently, most preparation techniques require an additional extrusion or filtering step to obtain monodisperse liposomes with the size of less than 100 nm. In this study, a compact liposome extruder was designed at a cost of $4.00 and used to synthesize liposome suspensions with defined particle size and high homogeneity for Murrayafoline A (Mu-A) loading and release. The synthesized MuA-loaded liposomes displayed a biphasic drug release and remained stable under the storage condition of 4°C. They also significantly reduced the viability of HepG2 cells in the cancer spheroids by 25%. The low-cost, flexible liposome extruder would allow the researchers to study liposomes and their applications in a cost-effective manner.

• Authors: Dan The Pham, Linh Phuong Nguyen, Quynh Thi Huong Pham, Chi Khanh Pham, Dung Thuy Nguyen Pham, Nguyen Thanh Viet, Hong Van Thi Nguyen, Toan Quoc Tran, Duong Thanh Nguyen
• Journal: Journal of biomaterials applications (J Biomater Appl) Vol. 37 Issue 5 Pg. 872-880 (11 2022) ISSN: 1530-8022 [Electronic] England
• PMID: 35786069 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Liposomes
• murrayafoline A
• Alkaloids
• Carbazoles

Topics

• Liposomes
• Drug Delivery Systems
• Alkaloids
• Carbazoles
• Particle Size
• Neoplasms (drug therapy)