Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.

Abstract

Metabolic disorders are closely associated with the dysregulation of circadian rhythms. Many bioactive components with lipid metabolism-regulating effects have been reported to function through circadian clock-related mechanisms. As the main pungent principle of black pepper, piperine (PIP) has been demonstrated to possess anti-obesity bioactivity by affecting hepatic lipid metabolism-related factors. However, whether the circadian clock genes Bmal1 and Clock are involved in the protective effect of PIP against lipid metabolism disorders remains unknown. In this work, oleic acid (OA) induced lipid accumulation in HepG2 cells. The effect of PIP on redox status, mitochondrial functions, and circadian rhythms of core clock genes were evaluated. Results revealed that PIP alleviated circadian desynchrony, ROS overproduction, and mitochondrial dysfunction. A mechanism study showed that PIP could activate the SREBP-1c/PPARγ and AMPK/AKT-mTOR signaling pathways in a Bmal1/Clock-dependent manner in HepG2 cells. These results indicated that Bmal1 and Clock played important roles in the regulating effect of PIP on hepatic lipid homeostasis.

• Authors: Weiyun Zhang, Chi-Tang Ho, Muwen Lu
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 23 Issue 10 (May 17 2022) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 35628429 (Publication Type: Journal Article)

Chemical References

• Alkaloids
• Benzodioxoles
• Lipids
• Piperidines
• Polyunsaturated Alkamides
• piperine

Topics

• Alkaloids
• Benzodioxoles (pharmacology)
• Hep G2 Cells
• Humans
• Lipids
• Piperidines
• Polyunsaturated Alkamides (pharmacology)