Pullulan nanofibers containing the antimicrobial palindromic peptide LfcinB (21-25)Pal obtained via electrospinning.

Abstract

Electrospinning technology is useful for making ultrafine drug-eluting fibers for the clinical treatment of wounds. We show the incorporation of an antimicrobial LfcinB-derived peptide into Pullulan nanofibers. The palindromic peptide LfcinB (21-25)Pal: RWQWRWQWR was synthesized, purified, and characterized by means of the RP-HPLC and MALDI-TOF MS methods. The peptide's antibacterial activity against the E. coli ATCC 25922 strain was evaluated, and the peptide LfcinB (20-25)Pal exhibited significant antibacterial activity. Nanofibers were obtained by electrospinning a Pullulan or Pullulan-LfcinB (21-25)Pal solution. The obtained nanofibers were characterized via microscopy (AFM and SEM) and RP-HPLC chromatography. The peptide incorporation efficiency was 31%. The Pullulan-LfcinB (21-25)Pal nanofibers were soluble in water, and the peptide was liberated immediately. The Pullulan-LfcinB (21-25)Pal nanofibers exhibited the same antibacterial activity against E. coli strain as the free peptide LfcinB (21-25)Pal. The results suggest that Pullulan-LfcinB (21-25)Pal nanofibers could be considered for designing and developing antibacterial wound dressings.

• Authors: Julieth Tatiana Román, Carlos Alberto Fuenmayor, Carlos Mario Zuluaga Dominguez, Dianney Clavijo-Grimaldo, Martha Acosta, Javier Eduardo García-Castañeda, Ricardo Fierro-Medina, Zuly Jenny Rivera-Monroy
• Journal: RSC advances (RSC Adv) Vol. 9 Issue 35 Pg. 20432-20438 (Jun 25 2019) ISSN: 2046-2069 [Electronic] England
• PMID: 35514725 (Publication Type: Journal Article)
• Copyright: This journal is © The Royal Society of Chemistry.