Abstract |
To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.
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Authors | Jieyu Wan, Fangjie Li, Mingshu Wang, Anchun Cheng, Bin Tian, Qiao Yang, Ying Wu, Xumin Ou, Sai Mao, Di Sun, Shaqiu Zhang, Juan Huang, Qun Gao, XinXin Zhao, Shun Chen, Mafeng Liu, Renyong Jia, Dekang Zhu |
Journal | Poultry science
(Poult Sci)
Vol. 101
Issue 6
Pg. 101863
(Jun 2022)
ISSN: 1525-3171 [Electronic] England |
PMID | 35489250
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved. |
Topics |
- Animals
- Chickens
- Ducks
- Mardivirus
- Morphogenesis
- Virion
- Virus Replication
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