Immunotherapy Mechanism of Esophageal Squamous Cell Carcinoma with the Effect of STK11/AMPK Signaling Pathway.

Abstract

This study was aimed at exploring the mechanism of serine threonine protein kinase 11 (STK11)/Adenosine 5'-monophosphate-activated protein kinase (AMPK) signaling pathway after immunotherapy for esophageal squamous cell carcinoma (ESCC), providing basic information for the clinical treatment of ESCC. In this study, tissue specimens from 100 patients with ESCC who underwent surgical treatment in Taizhou People's Hospital (group A) and 20 patients with recurrent or metastatic ESCC who received second-line immunotherapy (group B) were collected. The real-time fluorescent quantitative polymerase chain reaction (PCR) (RT-qPCR) technology was used to detect the expression levels of STK11, interferon-γ (IFN-γ), interleukin 6 (IL-6), and vascular endothelial growth factor (VEGF) in the tissues. The immunohistochemical staining was used to detect the positive expression levels (PELs) of STK11 and AMPKα in the tissues, and immunofluorescence staining was used to detect the PELs Teff cells (CD3 and CD8), Treg cells (CD4 and FOXP3), and neutrophils (CD68 and CD163). RT-qPCR results showed that the expression levels of STK11 and IFN-γ in group A were obviously lower, and those of IL-6 and VEGF were much higher in contrast to group B (P < 0.05). The results of immunohistochemical staining showed that the number of STK11- and AMPKα-positive staining cells in group A was dramatically less than that in group B (P <0.05). The results of immunofluorescence staining revealed that the number of positive staining cells for Teff cells, Treg cells, and neutrophils in group A was also less dramatically than that in group B (P <0.05). In summary, immunotherapy can play a therapeutic effect on ESCC by regulating STK11/AMPK pathway and immune cell infiltration.

• Authors: Yang Xia, Peng Wang, Yunyao Ye, Sihui Zhang, Guangzhi Sun, Jie Xu, Gaohua Han
• Journal: BioMed research international (Biomed Res Int) Vol. 2022 Pg. 8636527 ( 2022) ISSN: 2314-6141 [Electronic] United States
• PMID: 35463992 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2022 Yang Xia et al.

Chemical References

• Interleukin-6
• Vascular Endothelial Growth Factor A
• Protein Serine-Threonine Kinases
• STK11 protein, human
• AMP-Activated Protein Kinase Kinases
• AMP-Activated Protein Kinases
• PRKAA1 protein, human

Topics

• AMP-Activated Protein Kinase Kinases (genetics, immunology)
• AMP-Activated Protein Kinases (genetics, immunology)
• Cell Line, Tumor
• Esophageal Neoplasms (genetics, immunology, surgery, therapy)
• Esophageal Squamous Cell Carcinoma (genetics, immunology, surgery, therapy)
• Gene Expression Regulation, Neoplastic
• Humans
• Immunotherapy (methods)
• Interleukin-6 (immunology)
• Protein Serine-Threonine Kinases (genetics)
• Signal Transduction
• Vascular Endothelial Growth Factor A (immunology)