Tamoxifen resistance remains a major obstacle in the treatment of
estrogen receptor (ER)‑positive
breast cancer. In recent years, the crucial role of the epithelial‑mesenchymal transition (EMT) process in the development of drug resistance in
breast cancer has been underlined. However, the central molecules inducing the EMT process during the development of
tamoxifen resistance remain to be elucidated. In the present study, it was demonstrated that tamoxifen‑resistant
breast cancer cells underwent EMT and exhibited an enhanced cell motility and invasive behavior. The inhibition of snail family transcriptional repressor 1 (Snail) and twist family BHLH transcription factor 1 (Twist) reversed the EMT phenotype and decreased the
tamoxifen resistance, migration and invasion of tamoxifen‑resistant
breast cancer cells. In addition, it was observed that the inhibition of
epidermal growth factor receptor (EGFR) reversed the EMT phenotype in tamoxifen‑resistant MCF7 (MCF‑7/TR) cells via the downregulation of Snail and Twist. Notably, the EGFR inhibitor,
gefitinib, decreased
tamoxifen resistance, migration and invasion through the inhibition of Snail and Twist. On the whole, the results of the present study suggest that EGFR may be a promising therapeutic target for tamoxifen‑resistant
breast cancer. Moreover, it was suggested that
gefitinib may serve as a potent novel therapeutic strategy for
breast cancer patients, who have developed
tamoxifen resistance.