Rapid detection of 5 fungal diseases in sunflower (Helianthus annuus) using dual priming oligonucleotide system-based multiplex PCR and capillary electrophoresis.

Abstract

Rapid and accurate diagnosis of fungal pathogens is essential for disease control in sunflower. In the present study, a multiplex PCR assay was developed based on the dual priming oligonucleotide (DPO) system, which was used to simultaneously detect and identify five major sunflower fungal pathogens. There was no cross-reactivity among the pathogens tested. In each reaction, 0.1 ng genomic DNA templates were sufficient to ensure specificity and accuracy. The system exhibited high adaptability over a wide range of annealing temperatures. No mismatch or nonspecific amplification was observed in the annealing temperatures tested. In combination with capillary electrophoresis, the DPO-primer-based multiplex PCR system provides a rapid, reliable and cost-efficient solution for the diagnosis of fungal pathogens in sunflower.

• Authors: Yike Qian, Shuang Wei, Na Zhang, Qianqian Yang, Weimin Chen, Xiaoqin Zhang, Huixia Zhao
• Journal: SLAS technology (SLAS Technol) Vol. 27 Issue 4 Pg. 253-260 (08 2022) ISSN: 2472-6311 [Electronic] United States
• PMID: 35367401 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022. Published by Elsevier Inc.

Chemical References

• Oligonucleotides

Topics

• Electrophoresis, Capillary
• Helianthus
• Multiplex Polymerase Chain Reaction (methods)
• Mycoses
• Oligonucleotides