Abstract |
Cervical squamous cell carcinoma (CSCC), the most common cervical malignancy, is more likely to invade and metastasize than other cervical cancers. miR-125a, a tumor suppressor gene, has been confirmed to be associated with cancer metastasis. However, the role of miR-125a in CSCC and the underlying mechanism are unknown. miR-125a expression was confirmed by real-time quantitative PCR (RT-qPCR), and the Rad51 expression level was measured by western blotting analysis. CSCC cell proliferation, migration and invasion were assessed with functional assays, including CCK-8, colony formation, wound healing and Transwell assays. Our data confirmed that miR-125a is expressed at low levels in CSCC tissues and cells. Functionally, the overexpression of miR-125a greatly prevented the proliferation, migration and invasion of CSCC cells, and the inhibition of miR-125a expression strongly enhanced these behaviors in CSCC cells. Moreover, the expression of Rad51, a miR-125a target gene, greatly reversed the miR-125-mediated inhibition of CSCC cell proliferation, migration and invasion. In addition, we discovered that miR-125a downregulated the levels of phosphorylated PI3K, AKT and mTOR through Rad51 in CSCC cells. miR-125a, a tumor suppressor, can attenuate the malignant behaviors of CSCC cells by targeting Rad51. Therefore, the miR-125a/Rad51 axis might be a target for CSCC therapy.
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Authors | Zeping Liu, Jinchang Huang, Qiuju Jiang, Xiaoling Li, Xiaohui Tang, Shasha Chen, Liling Jiang, Genghua Fu, Sijun Liu |
Journal | Bioengineered
(Bioengineered)
Vol. 13
Issue 4
Pg. 8503-8514
(04 2022)
ISSN: 2165-5987 [Electronic] United States |
PMID | 35332852
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MIRN125 microRNA, human
- MicroRNAs
- RAD51 protein, human
- Rad51 Recombinase
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Topics |
- Carcinoma, Squamous Cell
(genetics)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Female
- Humans
- MicroRNAs
(genetics)
- Rad51 Recombinase
(genetics)
- Uterine Cervical Neoplasms
(genetics)
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