ALG3 Is a Potential Biomarker for the Prognosis of Bladder Cancer.

Abstract

OBJECTIVE:

Previous research showed that ALG3 was associated with several cancers, but the function of ALG3 in bladder cancer (BC) was yet unknown. The purpose of this study was to investigate the relative expression of ALG3 in BC tissues and corresponding normal tissues and the relationship between the relative expression of ALG3 and clinical outcome in bladder cancer patients.

METHODS:

In this study, the expression of ALG3 in bladder cancer was detected by immunochemistry. In order to determine the cell proliferation and migration ability more accurately, we performed colony forming assay, MTT assay and wound healing migration assay. The role of ALG3 on tumor growth and metastasis was explored by animal model in vivo.

RESULTS:

ALG3 was expressed higher in bladder cancer than that in the normal tissues (P<0.05). At the same time, we found that there was a positive correlation between ALG3 expression and the prognosis (P<0.05). Moreover, we also discovered that the expression of ALG3 was associated with clinical pathological features (P<0.05). The proliferation and migration abilities of bladder cancer cell line T24 and 5637 were inhibited by silencing ALG3. In addition, the growth of bladder cancer cell line T24 cells were inhibited by silencing ALG3 in vivo.

CONCLUSION:

Silencing ALG3 plays a critical role in bladder cancer development and growth. It inhibits bladder cancer cells growth in vitro and in vivo.

• Authors: Ming Li, Ning Zhang, Weimin Shan, Bo Guan
• Journal: Annals of clinical and laboratory science (Ann Clin Lab Sci) Vol. 52 Issue 1 Pg. 117-125 (Jan 2022) ISSN: 1550-8080 [Electronic] United States
• PMID: 35181625 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 by the Association of Clinical Scientists, Inc.

Chemical References

• Biomarkers, Tumor
• ALG3 protein, human
• Mannosyltransferases

Topics

• Animals
• Biomarkers, Tumor (metabolism)
• Cell Line, Tumor
• Cell Movement (physiology)
• Cell Proliferation (physiology)
• Humans
• Mannosyltransferases (metabolism)
• Prognosis
• Urinary Bladder Neoplasms (enzymology, metabolism, pathology)