Ginsenoside Rg2 Ameliorating CDAHFD-Induced Hepatic Fibrosis by Regulating AKT/mTOR-Mediated Autophagy.

Abstract

Ginsenoside Rg2 (G-Rg2) in the rhizome of Panax ginseng can modify lipid accumulation, oxidative stress, and apoptosis in the liver induced by a high-fat diet. This research adds to this by assessing the potential antifibrosis effect of G-Rg2 (including possible mechanisms). G-Rg2 significantly improved pathological changes in liver tissue induced by a choline-deficient, l-amino acid-defined, high-fat diet (CDAHFD), it inhibited serum transaminase, plasma lipopolysaccharide, and liver hydroxyproline levels; it inhibited TGF-β1, α-SMA, and COL1A1 expression, it activated the AKT/mTOR signal pathway, and it inhibited liver expression of autophagy-related proteins. The in vitro experiments showed that G-Rg2 also restored the autophagy flux impairment induced by oleic acid and inhibited TGF-β1 expression by promoting p62 degradation in hepatocytes. In hepatic stellate (HSC-T6) cells, G-Rg2 reversed lipopolysaccharide-induced activation through the AKT/mTOR signaling pathway, inhibiting autophagy. Thus, G-Rg2 ameliorates CDAHFD-induced liver fibrosis and lipopolysaccharide-induced HSC-T6 cell activation by inhibiting AKT/mTOR-mediated autophagy.

• Authors: Ziwei He, Siyu Chen, Tingting Pan, Ao Li, Kangyu Wang, Zhuofeng Lin, Wei Liu, Yi Wang, Yanfang Wang
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 70 Issue 6 Pg. 1911-1922 (Feb 16 2022) ISSN: 1520-5118 [Electronic] United States
• PMID: 35104139 (Publication Type: Journal Article)

Chemical References

• Ginsenosides
• ginsenoside Rg2
• MTOR protein, human
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases

Topics

• Autophagy
• Ginsenosides
• Hepatic Stellate Cells
• Humans
• Liver Cirrhosis (drug therapy, genetics)
• Proto-Oncogene Proteins c-akt (genetics)
• TOR Serine-Threonine Kinases (genetics)