Magnolol-loaded cholesteryl biguanide conjugate hydrochloride nanoparticles for triple-negative breast cancer therapy.

Abstract

The potential of combination therapy using nanoparticle delivery systems in improving triple-negative breast cancer treatment efficacy remains to be explored. Here, we report a novel nanoparticle system using a cholesterol biguanide conjugate hydrochloride (CBH) as both a drug and carrier to load magnolol (MAG). Poly(ethylene glycol)-poly(lactic-co-glycolic acid) (mPEG-PLGA) and aminoethyl anisamide-poly(ethylene glycol)-poly(lactic-co-glycolic acid) (AEAA-PEG-PLGA) were added to form nanoparticles. Nanoparticles accumulated most in tumor tissues when the weight ratio of AEAA-PEG-PLGA to mPEG-PLGA was 4:1. MAG and CBH exerted a synergistic inhibitory effect on 4 T1 cells. An in vitro study showed that nanoparticles displayed the highest tumor cell uptake rate, highest apoptosis rate, and strongest inhibitory effect on tumor cell migration and monoclonal formation. CBH might promote nanoparticle uptake by cells and lysosomal escape. After intravenous administration to mice with 4 T1 breast tumors in situ, the nanoparticles inhibited tumor growth without obvious toxicity. Western blot results showed that nanoparticles altered the levels of p53, p-AKT, and p-AMPK in the tumor tissue. Moreover, cell apoptosis was found in the same area of H&E-stained and TUNEL-stained tumors treated with the nanoparticles. Collectively, this nanoparticle system provides a novel combination drug delivery strategy for treating triple-negative breast cancer.

• Authors: Yanzhi Wang, Cancan Sun, Leaf Huang, Mengqian Liu, Lu Li, Xiping Wang, Linchao Wang, Shanshan Sun, Haiwei Xu, Gege Ma, Lei Zhang, Jiaxin Zheng, Hongmin Liu
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 615 Pg. 121509 (Mar 05 2022) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 35085734 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Biguanides
• Biphenyl Compounds
• Drug Carriers
• Lignans
• magnolol
• Polyethylene Glycols

Topics

• Animals
• Biguanides
• Biphenyl Compounds
• Cell Line, Tumor
• Drug Carriers
• Humans
• Lignans
• Mice
• Nanoparticles
• Polyethylene Glycols
• Triple Negative Breast Neoplasms (drug therapy)