Abstract | CONTEXT: OBJECTIVE: To explore whether DAS exerts an antithrombotic effect and its internal mechanism. MATERIALS AND METHODS: Human blood samples and Sprague-Dawley (SD) rats divided into control, aspirin (30 mg/kg), and DAS groups (200, 400 and 600 mg/kg) were used to measure the platelet aggregation rate, coagulation function, coagulation factor activity, and contents of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α). The histopathology of the SD rat gastric mucosa was also observed. All rats were administered intragastric or intraperitoneal injections once a day for 3 consecutive days. RESULTS: Compared to control group, DAS significantly inhibited the platelet aggregation rate (ED50 = 386.9 mg/kg) by decreasing TXB2 levels (1531.95 ± 649.90 pg/mL to 511.08 ± 411.82 pg/mL) and activating antithrombin III (AT-III) (103.22 ± 16.22% to 146.46 ± 8.96%) (p < 0.05). In addition, DAS significantly enhanced the coagulation factors FV (304.12 ± 79.65% to 443.44 ± 75.04%), FVII (324.19 ± 48.03% to 790.66 ± 225.56%), FVIII (524.79 ± 115.47% to 679.92 ± 143.34%), FX (34.90 ± 7.40% to 102.76 ± 29.41%) and FXI (38.12 ± 10.33% to 65.47 ± 34.08%), increased the content of Fg (2.18 ± 0.39 to 3.61 ± 0.37 g/L), shorten the PT (10.42 ± 0.44 to 9.22 ± 0.21 s), APTT (16.43 ± 1.4 to 14.07 ± 0.75 s) and TT time (37.04 ± 2.13 to 32.68 ± 1.29 s) (p < 0.05), while the aspirin group showed no such effect on these items but showed reduced activity of FII (89.21 ± 21.72% to 61.83 ± 8.95%) and FVIII (524.79 ± 115.47% to 306.60 ± 29.96%) (p < 0.05). Histopathological changes showed aspirin-induced gastric mucosa haemorrhage and the protective effect of DAS in the gastric mucosa. CONCLUSIONS:
DAS is more suitable than aspirin in thromboprophylaxis treatment, which provides a reliable theoretical and experimental basis for its clinical application.
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Authors | Bowen Yin, Shuhua Zhang, Yuxi Huang, Yuanzhu Long, Yiguo Chen, Shiyun Zhao, Aiqun Zhou, Minghua Cao, Xiaoming Yin, Daya Luo |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 60
Issue 1
Pg. 175-184
(Dec 2022)
ISSN: 1744-5116 [Electronic] England |
PMID | 35014931
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Diterpenes
- Fibrinolytic Agents
- Platelet Aggregation Inhibitors
- Succinates
- Aspirin
- dehydroandrographolide
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Topics |
- Animals
- Aspirin
(adverse effects, pharmacology)
- Blood Coagulation
(drug effects)
- Diterpenes
(administration & dosage, pharmacology)
- Dose-Response Relationship, Drug
- Female
- Fibrinolytic Agents
(administration & dosage, pharmacology)
- Gastric Mucosa
(drug effects, pathology)
- Humans
- Male
- Middle Aged
- Platelet Aggregation
(drug effects)
- Platelet Aggregation Inhibitors
(administration & dosage, adverse effects, pharmacology)
- Rats
- Rats, Sprague-Dawley
- Succinates
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