Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.

Abstract

Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy. Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata. Monosaccharide composition indicated that the AQP70-2B was composed of rhamnose, glucose, galactose, and arabinose. The backbone of AQP70-2B consisted of →1)-l-Araf, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, →4)-d-Glcp-(1→, →6)-d-Galp-(1→, and →1)-d-Rhap residues. Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B. Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized. Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.

• Authors: Huimei Wang, Ying Li, Xuelian Wang, Yuhao Li, Jianlin Cui, Da-Qing Jin, Muhetaer Tuerhong, Munira Abudukeremu, Jing Xu, Yuanqiang Guo
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 278 Pg. 118950 (Feb 15 2022) ISSN: 1879-1344 [Electronic] England
• PMID: 34973766 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier Ltd. All rights reserved.

Chemical References

• Angiogenesis Inhibitors
• Antineoplastic Agents, Phytogenic
• Polysaccharides
• Selenium

Topics

• Angiogenesis Inhibitors (chemistry, isolation & purification, pharmacology)
• Animals
• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
• Carbohydrate Conformation
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Drug Screening Assays, Antitumor
• Fruit (chemistry)
• Hep G2 Cells
• Humans
• Liver Neoplasms, Experimental (drug therapy, pathology)
• Neovascularization, Pathologic (drug therapy, pathology)
• Polysaccharides (chemistry, isolation & purification, pharmacology)
• Ranunculales (chemistry)
• Selenium (chemistry)
• Zebrafish (embryology)