Abstract |
At present, there is no effective noninvasive method for the accurate diagnosis of early-stage lung adenocarcinoma (LUAD). This study examined the profile of plasma extracellular vesicle (EV)-delivered microRNAs ( miRNAs) in patients with invasive stage I LUAD. In this study, a total of 460 participants were enrolled, including 254 patients with LUAD, 76 patients with benign pulmonary nodules (BPNs), and 130 healthy control patients (HCs). miRNA sequencing was used to analyze the EV miRNA profile of the patient plasma samples (n = 150). A diagnostic signature (d-signature) was identified by applying a stepwise logistic regression algorithm, and a single-center training cohort (n = 150) was tested, followed by a multicenter validation cohort (n = 100). A d-signature comprising four EV-derived miRNAs (hsa-miR-106b-3p, hsa-miR-125a-5p, hsa-miR-3615, and hsa-miR-450b-5p) was developed for the early detection of LUAD. The d-signature had high precision with area under the curve (AUC) values of 0.917 and 0.902 in the training and test cohorts, respectively. Moreover, the d-signature could recognize patients with adenocarcinoma in situ (AIS) and minimally invasive adenocarcinoma (MIA) with AUC values of 0.846 and 0.92, respectively. To sum up, our study detailed the plasma EV-derived miRNA profile in early LUAD patients and developed an EV-derived miRNA d-signature to detect early LUAD.
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Authors | Shugeng Gao, Wei Guo, Tiejun Liu, Naixin Liang, Qianli Ma, Yibo Gao, Fengwei Tan, Qi Xue, Jie He |
Journal | Cancer science
(Cancer Sci)
Vol. 113
Issue 2
Pg. 648-659
(Feb 2022)
ISSN: 1349-7006 [Electronic] England |
PMID | 34837453
(Publication Type: Journal Article, Multicenter Study)
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Copyright | © 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. |
Chemical References |
- Biomarkers, Tumor
- MicroRNAs
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Topics |
- Adenocarcinoma of Lung
(blood, diagnosis, genetics, pathology)
- Adult
- Biomarkers, Tumor
(blood, genetics)
- Diagnosis, Differential
- Extracellular Vesicles
(genetics)
- Female
- Humans
- Lung Neoplasms
(blood, diagnosis, genetics, pathology)
- Male
- MicroRNAs
(blood, genetics)
- Middle Aged
- Neoplasm Staging
- Prospective Studies
- Reproducibility of Results
- Sensitivity and Specificity
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