Abstract |
Perfluorooctane sulfonic acid (PFOS) is a persistent environmental pollutant. Exposure to PFOS has been associated with abnormal fetal development. The long non-coding RNA ( lncRNA) has been showed to play a role in fetal growth restriction (FGR), preeclampsia (PE) and other pregnancy complications. Whether the lncRNA contributes to PFOS-induced toxicity in the placenta remains unknown. In this study, we investigated the function of lncRNA MEG3 and its derived miR-770 in PFOS-induced placental toxicity. Pregnant mice received gavage administration of different concentrations of PFOS (0.5, 2.5, and 12.5 mg/kg/day) from GD0 to GD17, and HTR-8/SVneo cells were treated with PFOS in the concentrations of 0, 10-1, 1, 10 μM. We found that expression levels of miR-770 and its host gene MEG3 were reduced in mice placentas and HTR-8/SVneo cells with exposure of PFOS. A significant hypermethylation was observed at MEG3 promoter in placentas of mice gestational-treated with PFOS. We also confirmed that MEG3 and miR-770 overexpression alleviated the cell growth inhibition induced by PFOS. Furthermore, PTX3 ( Pentraxin 3) was identified as the direct target of miR-770 and it was enhanced after PFOS exposure. In summary, our results suggested that MEG3 alleviate PFOS-induced placental cell inhibition through MEG3/miR-770/PTX3 axis.
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Authors | Jing Li, Xiaojie Quan, Saifei Lei, Gang Chen, Jiawei Hong, Zhenyao Huang, Qi Wang, Weiyi Song, Xinxin Yang |
Journal | Environmental pollution (Barking, Essex : 1987)
(Environ Pollut)
Vol. 293
Pg. 118542
(Jan 15 2022)
ISSN: 1873-6424 [Electronic] England |
PMID | 34801623
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier Ltd. All rights reserved. |
Chemical References |
- Alkanesulfonic Acids
- Fluorocarbons
- MEG3 non-coding RNA, mouse
- MIRN770 microRNA, mouse
- MicroRNAs
- Nerve Tissue Proteins
- RNA, Long Noncoding
- neuronal pentraxin
- C-Reactive Protein
- perfluorooctane sulfonic acid
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Topics |
- Alkanesulfonic Acids
(toxicity)
- Animals
- C-Reactive Protein
- Female
- Fluorocarbons
(toxicity)
- Mice
- MicroRNAs
(genetics)
- Nerve Tissue Proteins
- Placenta
- Pregnancy
- RNA, Long Noncoding
(genetics)
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