Abstract | STATEMENT OF PROBLEM: As the cobalt chromium (Co-Cr) powder used in selective laser melting (SLM) is costly, reusing the remaining powder after multiple cycles provides an economic and environmental benefit. However, knowledge of the cytotoxic effect of the alloy fabricated from recycled powder is lacking. PURPOSE: The purpose of this in vitro study was to evaluate the biological effects of the Co-Cr ions released from the alloy fabricated from the recycled powder on the human gingival fibroblasts (HGFs) and normal oral keratinocytes (NOKs). MATERIAL AND METHODS: Disk-shaped Co-Cr specimens were fabricated by using the SLM technique from powders with different proportions of recycled to unused and from different recycling times. Co and Cr ions released from the disks immersed in the Dulbecco Modified Eagle Medium (DMEM) for 24 hours or 7 days were measured by inductively coupled plasma mass spectrometry (ICP-MS). Biocompatibility of Co-Cr alloy was detected by incubation of HGFs and NOKs in DMEM containing Co and Cr ions for 24 hours. The ANOVA test was used to evaluate statistically significant differences among different groups (α=.05). RESULTS: CONCLUSIONS: The results of this study indicated that Co-Cr alloy fabricated from partially recycled powder or powder with different recycling times released significantly more Co and Cr ions and showed higher cytotoxicity to HGFs and NOKs than the alloy fabricated from unused powder.
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Authors | Taghrid Aldhohrah, Jiajun Yang, Jiawen Guo, Hui Zhang, Yan Wang |
Journal | The Journal of prosthetic dentistry
(J Prosthet Dent)
Vol. 130
Issue 3
Pg. 393-401
(Sep 2023)
ISSN: 1097-6841 [Electronic] United States |
PMID | 34782150
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Powders
- Cobalt
- Chromium
- Vascular Endothelial Growth Factor A
- Chromium Alloys
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Topics |
- Humans
- Powders
- Cobalt
(chemistry)
- Chromium
(chemistry)
- Vascular Endothelial Growth Factor A
- Materials Testing
- Chromium Alloys
(chemistry)
- Lasers
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