Clinical significance of microRNA-1180-3p for colorectal cancer and effect of its alteration on cell function.

Abstract

An early diagnosis and effective prognostic factors would greatly reduce the mortality rate of colorectal cancer (CRC). This research is intended to complete the evaluation of the prognostic value and potential role of miR-1180-3p in CRC. The miR-1180-3p levels were reduced in CRC patients' tissues, blood, and human CRC cell lines. The ability of miR-1180-3p was explored in discrimination of CRC patients and healths and the value in overall survival estimate. The effect of miR-1180-3p dysregulation on the CRC cellular function was investigated. miR-1180-3p is downregulated in CRC tissues, blood and cells than normal ones. This lower expression was correlated with vascular invasion, lymph node metastasis, and TNM stage. With the use of ROC curve, miR-1180-3p showed discriminating ability in CRC patients and healthy subjects. With the result of Kaplan-Meier analysis and multi-multivariate Cox analysis, miR-1180-3p was an independent predictor for CRC patients' overall survival. Utilizing CCK-8, Transwell and matrigel assays, overexpression of miR-1180-3p reduced cancer cell proliferation and mobility, but induced apoptosis, by targeting COL12A1. miR-1180-3p might function as a suppressor in CRC progression and allowed the discovery of a new biomarker for diagnosis, prognosis and therapy target for CRC.

• Authors: Chunlei Li, Wei Jin, Dewei Zhang, Shoujin Tian
• Journal: Bioengineered (Bioengineered) Vol. 12 Issue 2 Pg. 10491-10500 (12 2021) ISSN: 2165-5987 [Electronic] United States
• PMID: 34723759 (Publication Type: Journal Article)

Chemical References

• COL12A1 protein, human
• Collagen Type XII
• MIRN1180 microRNA, human
• MicroRNAs

Topics

• Apoptosis (genetics)
• Base Sequence
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Collagen Type XII (metabolism)
• Colorectal Neoplasms (blood, genetics, pathology)
• Down-Regulation (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Kaplan-Meier Estimate
• Male
• MicroRNAs (blood, genetics, metabolism)
• Middle Aged
• Multivariate Analysis
• ROC Curve
• Risk Factors
• Survival Analysis