Long non-coding RNA (LncRNA) SNHG7/ Eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) involves in the malignant events of ovarian cancer cells with paclitaxel resistant.

Abstract

LncRNA SNHG7 shows a strong relationship with malignant behavior of cancer cells and poor clinical outcome in cancer. The resistance of ovarian cancer for Paclitaxel seriously limits the clinical efficacy in chemotherapy for ovarian cancer patients. In this study, we investigated whether lncRNA SNHG7 was involved in Paclitaxel sensitivity of ovarian cancer as well as the underlying mechanism regulating the behavior of ovarian cancer cells with Paclitaxel resistance. The experiment results of wound healing and transwell showed that in paclitaxel-resistant ovarian cancer cells, transfection with siRNA-SNHG7 in ovarian cancer cells reduced cell migration and invasion. And cell cycle was observed by means of Flow cytometry. RNA immunoprecipitation assay was performed to analyze the interaction of lncRNA SNHG7 and EIF4G2. Overexpression of EIF4G2 by transfection with Ov- EIF4G2 plasmids efficiently blocked the changes of migration and invasion, as well as G0/1 arrest caused by lncRNA SNHG7 silencing. Taken together, these results demonstrated that lncRNA SNHG7 could affect the degradation of EIF4G2 to regulate the sensitivity of ovarian cancer to Paclitaxel, inhibit cell viability, migration, and invasion. The interaction of lncRNA SNHG7 and EIF4G2 plays an important role in the migrative and invasive activity and Paclitaxel resistance of ovarian cancer cells.

• Authors: Jin Zhang, Rui Zhang, Yongju Ye
• Journal: Bioengineered (Bioengineered) Vol. 12 Issue 2 Pg. 10541-10552 (12 2021) ISSN: 2165-5987 [Electronic] United States
• PMID: 34709112 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• EIF4G2 protein, human
• Eukaryotic Initiation Factor-4G
• RNA, Long Noncoding
• RNA, Small Interfering
• Tumor Suppressor Protein p53
• PTEN Phosphohydrolase
• Paclitaxel

Topics

• Cell Cycle Checkpoints (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects, genetics)
• Cell Survival (drug effects, genetics)
• Drug Resistance, Neoplasm (drug effects, genetics)
• Eukaryotic Initiation Factor-4G (metabolism)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Gene Silencing
• Humans
• Neoplasm Invasiveness
• Ovarian Neoplasms (genetics, pathology)
• PTEN Phosphohydrolase (metabolism)
• Paclitaxel (pharmacology)
• RNA, Long Noncoding (genetics, metabolism)
• RNA, Small Interfering (metabolism)
• Tumor Suppressor Protein p53 (metabolism)