Down-expression of the NLRP3 inflammasome delays the progression of diabetic retinopathy.

Abstract

The investigation aimed to evaluate the effects of Mcc950, an inhibitor of the NLRP3 inflammasome, on diabetic retinopathy (DR) mice. The general physiological condition of each group of mice was recorded. Retinal blood vessels were stained for observation of the density of blood vessels, and retinas were used for further morphological examination and fluorescent staining after the intravitreal injection of Mcc950. Mcc950 partially reversed hyperglycemia-induced vascular damage and had reduced histological changes compared to DR mice. IL-1β production in mice retinas in the diabetic model (DM) group increased, but pretreatment with Mcc950 significantly reversed these changes. Additionally, Mcc950 engineered reduced FITC dextran extravasation and vascular leakage. Therefore, it played an apparent protective role in DR and could be a new treatment strategy for DR.

• Authors: Keke Ge, Yatao Wang, Pan Li, Miao Li, Wenhua Zhang, Handong Dan, Xumeng Hu, Jiamu Zhou, Qixiang Yang, Jiaojiao Wang, Zongming Song
• Journal: Microvascular research (Microvasc Res) Vol. 139 Pg. 104265 (01 2022) ISSN: 1095-9319 [Electronic] United States
• PMID: 34662588 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021. Published by Elsevier Inc.

Chemical References

• Anti-Inflammatory Agents
• Blood Glucose
• Furans
• IL1B protein, mouse
• Indenes
• Inflammasomes
• Inflammation Mediators
• Interleukin-1beta
• NLR Family, Pyrin Domain-Containing 3 Protein
• Nlrp3 protein, mouse
• Sulfonamides
• N-(1,2,3,5,6,7-hexahydro-S-indacen-4-ylcarbamoyl)-4-(2-hydroxy-2-propanyl)-2-furansulfonamide

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage, pharmacology)
• Blood Glucose (metabolism)
• Capillary Permeability (drug effects)
• Diabetic Retinopathy (immunology, metabolism, pathology, prevention & control)
• Disease Models, Animal
• Disease Progression
• Furans (administration & dosage, pharmacology)
• Indenes (administration & dosage, pharmacology)
• Inflammasomes (antagonists & inhibitors, metabolism)
• Inflammation Mediators (metabolism)
• Interleukin-1beta (metabolism)
• Intravitreal Injections
• Male
• Mice, Inbred C57BL
• NLR Family, Pyrin Domain-Containing 3 Protein (antagonists & inhibitors, metabolism)
• Retinal Neovascularization (immunology, metabolism, pathology, prevention & control)
• Retinal Vessels (drug effects, immunology, metabolism, pathology)
• Signal Transduction
• Sulfonamides (administration & dosage, pharmacology)