LncRNA SNHG3 is responsible for the deterioration of colorectal carcinoma through regulating the miR-370-5p/EZH1 axis.

Abstract

OBJECTIVE:

To illustrate the biological function of long non-coding RNA (lncRNA) SNHG3 in the deterioration of colorectal cancer (CRC) by regulating the miR-370-5p/EZH1 axis.

PATIENTS AND METHODS:

SNHG3 levels in fifty pairs of CRC and non-tumor tissues were examined by quantitative real-time polymerase chain reaction (qRT-PCR). Its correlation to tumor staging, lymph node metastasis and prognosis of CRC was analyzed. Cell counting kit-8 (CCK-8) and 5-Ethynyl-2'- deoxyuridine (EdU) assay were conducted to assess the influence of SNHG3 on CRC cell proliferation in vitro. In addition, invasive ability of CRC cells transfected with si-SNHG3 was explored by transwell assay. The binding and regulatory relations in the SNHG3/miR-370-5p/EZH1 axis were ascertained by Dual-Luciferase reporter assay.

RESULTS:

SNHG3 was upregulated in CRC tissues and cell lines. Its high level was correlated to advanced tumor staging, positive lymph node metastasis and poor prognosis of CRC. Knockdown of SNHG3 reduced proliferative and invasive rates of SW480 and HT29 cells. The SNHG3/miR-370-5p/EZH1 axis was ascertained. In addition, knockdown of miR-370-5p enhanced proliferative and invasive rates of SW480 and HT29 cells.

CONCLUSIONS:

LncRNA SNHG3 induces proliferative and invasive potentials of CRC by regulating the miR-370-5p/EZH1 axis.

• Authors: Y Zhang, L Li, K-X Lu, L-B Yu, J Meng, C-Y Liu
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 25 Issue 19 Pg. 6131-6137 (Oct 2021) ISSN: 2284-0729 [Electronic] Italy
• PMID: 34661273 (Publication Type: Journal Article)

Chemical References

• MIRN370 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• EZH1 protein, human
• Polycomb Repressive Complex 2

Topics

• Cell Line, Tumor
• Cell Proliferation (genetics)
• Colorectal Neoplasms (genetics, pathology)
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• HT29 Cells
• Humans
• Lymphatic Metastasis (genetics)
• MicroRNAs (genetics)
• Neoplasm Staging
• Polycomb Repressive Complex 2 (genetics)
• Prognosis
• RNA, Long Noncoding (genetics)
• Up-Regulation