Abstract | AIMS: Swietenia macrophylla have been considered for the treatment of various diseases, including anticancer activity. This study aimed to investigate the anticancer activity of S. macrophylla leaves extract and its isolated compound towards human colorectal cancer cell line. MAIN METHODS: Hexanic extract of S. macrophylla leaves demonstrated relevant cytotoxicity only against colon cancer cell line HCT116. KEY FINDINGS: Our results showed significant DNA damage and apoptosis after treatment with the hexanic extract of S. macrophylla. Moreover, no toxicity was noticed for the animal model. The isolated compound limonoid L1 showed potent cytotoxicity against cancer cell lines with IC50 at 55.87 μg mL-1. Limonoid L1 did not trigger any cell membrane rupture in the mice erythrocytes suggesting no toxicity. The antiproliferative effect of L1 was confirmed in colorectal cancer cells by clonogenic assay, inducing G2/M arrest, apoptosis, and DNA damage in cancer-type cells. SIGNIFICANCE:
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Authors | Laine C Pinto, Felipe P Mesquita, Leilane H Barreto, Pedro F N Souza, Ingryd N F Ramos, Adrielly V U Pinto, Bruno M Soares, Milton N da Silva, Rommel M R Burbano, Raquel C Montenegro |
Journal | Life sciences
(Life Sci)
Vol. 285
Pg. 119949
(Nov 15 2021)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 34543640
(Publication Type: Journal Article)
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Copyright | Copyright © 2021. Published by Elsevier Inc. |
Chemical References |
- Antineoplastic Agents
- Limonins
- Plant Extracts
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Colorectal Neoplasms
(metabolism, pathology)
- DNA Damage
- Erythrocytes
(drug effects)
- Female
- G2 Phase Cell Cycle Checkpoints
(drug effects)
- HCT116 Cells
- Hemolysis
- Humans
- Limonins
(isolation & purification, pharmacology, therapeutic use)
- Meliaceae
(chemistry)
- Mice
- Plant Extracts
(chemistry, pharmacology)
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