Abstract |
Campylobacter jejuni infection is a leading cause of foodborne disease, common to children, adult travelers, and military populations in low- to middle-income countries. In the absence of a licensed vaccine, efforts to evaluate prophylactic agents are underway. The prophylactic efficacy of a twice-daily, 550 mg dose of the antibiotic rifaximin demonstrated no efficacy against campylobacteriosis in a controlled human infection model (CHIM); however, samples from the CHIM study were utilized to assess how the human gut microbiome responds to C. jejuni infection, and if a 'protective' microbiota exists in study participants not developing campylobacteriosis. Statistically significant, but minor, differences in study participant beta diversity were identified during the challenge period (p = 0.002, R2 = 0.042), but no significant differences were otherwise observed. Pre-challenge alpha diversity was elevated in study participants who did not develop campylobacteriosis compared to those who did (p < 0.001), but alpha diversity declined in all study participants from the pre-challenge period to post-discharge. Our work provides insight into gut microbiome shifts observed during a C. jejuni CHIM and following antibiotic treatment. This study utilized a high dose of 1.7 x 105 colony-forming units of C. jejuni; future work could include CHIM studies performed with inocula more closely mimicking natural exposure as well as field studies involving naturally-occurring enteric infections.
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Authors | Blake W Stamps, Janelle Kuroiwa, Sandra D Isidean, Megan A Schilling, Clayton Harro, Kawsar R Talaat, David A Sack, David R Tribble, Alexander C Maue, Joanna E Rimmer, Renee M Laird, Chad K Porter, Michael S Goodson, Frédéric Poly |
Journal | Frontiers in cellular and infection microbiology
(Front Cell Infect Microbiol)
Vol. 11
Pg. 702047
( 2021)
ISSN: 2235-2988 [Electronic] Switzerland |
PMID | 34532299
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2021 Stamps, Kuroiwa, Isidean, Schilling, Harro, Talaat, Sack, Tribble, Maue, Rimmer, Laird, Porter, Goodson and Poly. |
Topics |
- Adult
- Aftercare
- Campylobacter Infections
- Campylobacter jejuni
- Child
- Gastrointestinal Microbiome
- Humans
- Patient Discharge
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