Circulating Cell-Free DNA-Based Detection of Tumor Suppressor Gene Copy Number Loss and Its Clinical Implication in Metastatic Prostate Cancer.

Abstract

Current liquid biopsy assays lack sufficient sensitivity to detect copy number loss, which limits the interrogation of critical tumor suppressor gene deletions during cancer progression and treatment. Here we describe a liquid biopsy assay with improved sensitivity for detection of copy number loss in blood samples with low levels of circulating tumor DNA, and demonstrate its utility by profiling PTEN, RB1, and TP53 genetic loss in metastatic prostate cancer patients.

• Authors: Xiaoxi Dong, Tiantian Zheng, Minhua Zhang, Chao Dai, Lili Wang, Lei Wang, Ruipeng Zhang, Yuan Long, Danyi Wen, Feng Xie, Yue Zhang, Yong Huang, Jianguo Dong, Huan Liu, Pan Du, Bonnie L King, Winston Tan, Shidong Jia, Chris X Lu, Manish Kohli, Haitao Wang, Jianjun Yu
• Journal: Frontiers in oncology (Front Oncol) Vol. 11 Pg. 720727 ( 2021) ISSN: 2234-943X [Print] Switzerland
• PMID: 34504797 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Dong, Zheng, Zhang, Dai, Wang, Wang, Zhang, Long, Wen, Xie, Zhang, Huang, Dong, Liu, Du, King, Tan, Jia, Lu, Kohli, Wang and Yu.