A novel circular RNA circPPFIA1 promotes laryngeal squamous cell carcinoma progression through sponging miR-340-3p and regulating ELK1 expression.

Abstract

Abnormal expression of circular RNA (circRNA) is closely related to the occurrence and development of many cancers. By screening the expression of circRNA, we identified a novel circRNA termed as has_circ_0023326 in laryngeal squamous cell carcinoma (LSCC). We verified the expression of circPPFIA1 and found that it was upregulated in LSCC tissues compared to the adjacent normal tissues. Functional studies were carried out to detect the effect of circPPFIA1 expression on the phenotype of LSCC cells. These results suggest that circPPFIA1 knockdown can suppress the proliferation, migration, and invasion of LSCC cells, while circPPFIA1 overexpression can promote these processes. Mechanistically, miR-340-3p was predicted to be the target miRNA sponged by circPPFIA1 as confirmed through the luciferase assay and rescue experiments. In addition, miR-340-3p was found to target ELK1 and inhibit its expression. Taken together, circPPFIA1 promotes the progression of LSCC via the miR-340-3p/ELK1 signaling axis, which may serve as a novel prognostic or therapeutic target for LSCC.

• Authors: Yu Shuang, Jing Liu, Juntao Niu, Wenyu Guo, Chao Li
• Journal: Bioengineered (Bioengineered) Vol. 12 Issue 1 Pg. 5220-5230 (12 2021) ISSN: 2165-5987 [Electronic] United States
• PMID: 34455918 (Publication Type: Journal Article)

Chemical References

• ELK1 protein, human
• MIRN340 microRNA, human
• MicroRNAs
• RNA, Circular
• ets-Domain Protein Elk-1

Topics

• Animals
• Cell Line, Tumor
• Disease Progression
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Laryngeal Neoplasms (genetics, metabolism, pathology)
• Mice
• MicroRNAs (genetics, metabolism)
• RNA, Circular (genetics, metabolism)
• Squamous Cell Carcinoma of Head and Neck (genetics, metabolism, pathology)
• ets-Domain Protein Elk-1 (genetics, metabolism)