Paeonol Suppresses Vasculogenesis Through Regulating Vascular Smooth Muscle Phenotypic Switching.

Abstract

OBJECTIVE:

Smooth muscle cell (SMC) phenotypic switching is associated with development of a variety of occlusive vascular diseases. Paeonol has been reported to be involved in suppressing SMC proliferation. However, it is still unknown whether paeonol can regulate SMC phenotypic switching, and which eventually result in suppressing vasculogenesis.

METHODS:

Murine left common carotid artery was injured by completely ligation, and paeonol was administrated by intraperitoneal injection. Hematoxylin and eosin (H&E) staining was performed to visualize vascular neointima formation. Rat aortic SMCs were used to determine whether paeonol suppresses cell proliferation and migration. And murine hind limb ischemia model was performed to confirm the function role of paeonol in suppressing vasculogenesis.

RESULTS:

Complete ligation of murine common carotid artery successfully induced neointima formation. Paeonol treatment dramatically reduced the size of injury-induced neointima. Using rat aortic primary SMC, we identified that paeonol strongly suppressed cell proliferation, migration, and decreased extracellular matrix deposition. And paeonol treatment dramatically suppressed vasculogenesis after hind limb ischemia injury.

CONCLUSION:

Paeonol could regulate SMC phenotypic switching through inhibiting proliferation and migration of SMC, which results in inhibiting ischemia-induced vasculogenesis.

• Authors: Huan Xu, Ziqiang Wu, Zhong Jin, Xiao Wu, Wangming Hu, Bing Liang, Guanhua Lou, Zixian Chen, Huan Yao, Xiongbing Chen, Xin Zhou, Han Xiao, Cenghao Yu, Delai Zhang, Daoying Gong, Lan Yang, Yaping Shi, Yiming Xu, Yong Wang
• Journal: Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists (J Endovasc Ther) Vol. 29 Issue 1 Pg. 117-131 (02 2022) ISSN: 1545-1550 [Electronic] United States
• PMID: 34355606 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetophenones
• paeonol

Topics

• Acetophenones
• Animals
• Carotid Artery Injuries (drug therapy)
• Cells, Cultured
• Mice
• Muscle, Smooth, Vascular
• Neointima
• Rats
• Treatment Outcome