HNRNPU-AS1 Regulates Cell Proliferation and Apoptosis via the MicroRNA 205-5p/AXIN2 Axis and Wnt/β-Catenin Signaling Pathway in Cervical Cancer.

Abstract

Long noncoding RNAs (lncRNAs) have key functions in modulating cervical cancer (CC) genesis and progression. This work focused on exploring lncRNA HNRNPU-AS1's function in CC and the underlying mechanism. HNRNPU-AS1, AXIN2, and microRNA 205-5p (miR-205-5p) levels in CC cases were measured through reverse transcription-quantitative PCR. The relationship between miR-205-5p and AXIN2 or HNRNPU-AS1 was validated through a dual-luciferase assay. Cell proliferation was examined by CCK-8 and cell apoptosis by colony formation and flow cytometry analysis. HNRNPU-AS1 expression loss could be observed in CC patients and cell lines, which predicted the dismal prognosis of CC cases. Moreover, it was identified that the miR-205-5p level was upregulated, which acted as an inhibitory target of HNRNPU-AS1 and AXIN2. HNRNPU-AS1 inhibited cell proliferation and promoted apoptosis. As revealed by Kaplan-Meier curve, CC cases showing low HNRNPU-AS1, high miR-205-5p, and low AXIN2 levels had the poorest prognosis. AXIN2 reversed the CC cell proliferation-promoting, apoptosis-inhibiting, and Wnt/β-catenin signaling-activating behavior mediated by miR-205-5p or HNRNPU-AS1 knockout. In conclusion, the overexpression of lncRNA HNRNPU-AS1 suppressed CC progression by inhibiting the Wnt/β-catenin pathway through the miR-205-5p/AXIN2 axis.

• Authors: Zhaoyuan Niu, Fengling Wang, Shihui Lv, Yingpin Lv, Ming Liu, Lei Fu, Yushuang Yao, Lingzhi Wang, Wei Lin, Fang Yuan
• Journal: Molecular and cellular biology (Mol Cell Biol) Vol. 41 Issue 10 Pg. e0011521 (09 24 2021) ISSN: 1098-5549 [Electronic] United States
• PMID: 34309414 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AXIN2 protein, human
• Axin Protein
• CTNNB1 protein, human
• HNRNPU protein, human
• Heterogeneous-Nuclear Ribonucleoprotein U
• MIRN205 microRNA, human
• MicroRNAs
• RNA, Antisense
• RNA, Long Noncoding
• beta Catenin

Topics

• Apoptosis (genetics)
• Axin Protein (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Female
• Gene Expression (genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Heterogeneous-Nuclear Ribonucleoprotein U (genetics)
• Humans
• MicroRNAs (genetics)
• Prognosis
• RNA, Antisense (genetics)
• RNA, Long Noncoding (genetics)
• Uterine Cervical Neoplasms (genetics, metabolism, physiopathology)
• Wnt Signaling Pathway (genetics)
• beta Catenin (genetics)